Cocrystallization of ubiquitin–deubiquitinase complexes through disulfide linkage

The crystal structures of two disulfide-linked ubiquitin-bound bacterial deubiquitinases reveal the mode of ubiquitin binding. These structures show that disulfide linking is an effective strategy for capturing covalent ubiquitin–deubiquitinase complex structures.


Introduction
Protein ubiquitination involves the post-translational covalent attachment of the 76-residue eukaryotic protein ubiquitin (Ub) to, most commonly, lysine residues of target proteins via an isopeptide bond that links the last glycine of Ub to the "-amino group of the target lysine.Attachment of a single Ub, or monoubiquitination, can serve as a regulatory signal, but protein targets are usually polyubiquitinated through successive extension of the Ub-Ub linkage using any of the seven lysines (or the Met1 �-amino group) of the modifier itself.In its simplest form, polyubiquitination can arise with eight topologically distinct linkage types in homotypic chains, which are chains produced by repeated use of the same Ub amino group.Additionally, the use of different lysines during chain extension, along with branching, can give rise to ubiquitination patterns that represent complex signals constituting what has been referred to as the ubiquitin code.The writers of the code are the E1, E2 and E3 enzymes, which function in coordination to first activate Ub by an ATP-requiring reaction catalyzed by the E1 enzyme, followed by transfer of the activated Ub to the catalytic cysteine of a ubiquitin-conjugating E2 enzyme and finally to a substrate lysine through the intermediacy of a Ub E3 ligase (Komander & Rape, 2012).The readers are one of several ubiquitin-binding domains found in receptor proteins that recognize the Ub tag and shepherd the modified protein to its biological fate (Husnjak & Dikic, 2012).Last but not least are erasers, or deubiquitinases (DUBs), that remove Ub from ubiquitinated proteins or trim and disassemble polyubiquitin chains, regulating the signal (Komander & Rape, 2012;Eletr & Wilkinson, 2014;Nijman et al., 2005).
The 90 or so eukaryotic DUBs can be classified into seven DUB families belonging to two broad mechanistic classes: cysteine proteases and metalloproteases (Komander & Rape, 2012;Eletr & Wilkinson, 2014;Nijman et al., 2005).Within the cysteine protease class, the eukaryotic DUBs are divided into six groups based on the structure of their catalytic domain: the ubiquitin carboxy-terminal hydrolase (UCH) family, the ubiquitin-specific protease (USP) family, the ovarian tumor (OTU) family, the Machado-Joseph domain (MJD) family, the motif interacting with Ub-containing novel DUB (MINDY) family and the zinc finger with UFM1-specific peptidase domain protein (zUFSP) family groups (Komander & Rape, 2012;Hewings et al., 2018;Kwasna et al., 2018), whereas the JAMM/MPN (JAMM) family of metalloenzymes are the only group of DUBs that use a zinc-dependent, thermolysin-like mechanism in their hydrolysis reaction (Sato et al., 2008;Shrestha et al., 2014).
Mechanistic understanding of DUBs requires their cocrystal structure with Ub to delineate the intermolecular recognition.A widely used strategy for capturing the Ubbound DUB complex relies on a Ub-derived irreversible inhibitor that carries a C-terminal electrophile warhead exactly in place of the scissile peptide bond immediately following Gly76, the site of DUB cleavage.The Ub moiety, captured through covalent tethering with the catalytic cysteine, invariably occupies the so-called distal site (or S1 site; Zhang & Das, 2023;Borodovsky et al., 2002).Ub at the proximal site (S1 0 site) has been captured using diubiquitin probes with a suitably placed internal electrophile between the Ub units (Li et al., 2014;Haj-Yahya et al., 2014).However, the synthesis of such probes requires special expertise.We reasoned that disulfide linking may provide an easy-to-access alternative that does not involve any specialized synthetic methods.For example, placement of cysteine in place of Ly63 of Ub should permit the capture of Ub in the S1 0 site of Lys63specific cysteine DUBs.Towards this goal, we sought to determine whether cysteine substitution and subsequent disulfide formation is feasible at all, which we wanted to test with the Gly76-to-Cys Ub mutant, which is meant to react with the catalytic cysteine and allow the capture of Ub at the S1 site of the DUB.
Here, we explored disulfide-bridge formation as a strategy for producing a stable DUB-Ub complex amenable to crystallization.We envisioned that placement of a cysteine residue in Ub, which otherwise lacks this residue in the native form, in place of the last glycine may permit covalent capture of DUB-Ub complexes, allowing structural characterization.To this end, the Gly76-to-Cys mutant of Ub (Ub G76C ) was tested for its ability to form a disulfide bond with the catalytic cysteine of a group of DUBs.Two previously crystallized complexes of bacterial DUBs from pathogenic organisms were subjected to crystallization as their Ub G76C complex, allowing an assessment of the disulfide strategy.While the SdeA DUB, an effector protein from the arsenal of Legionella pneumophila (Sheedlo et al., 2015(Sheedlo et al., , 2021)), reacted with the Ub cysteine in the expected manner showing Ub binding at the S1 site, the effector DUB from Orientia tsutsugamushi (OtDUB; Berk et al., 2020) preferentially reacted with a noncatalytic cysteine.

Protein expression and purification
OtDUB 1-259 cloned into a pET-28a vector was obtained from Mark Hochtrasser (Yale University) and transformed into Escherichia coli strain BL21(DE3) (Novagen).The E. coli BL21(DE3) cells were grown in lysogeny broth (LB) medium at 37 � C to an OD 600 of 0.6, cooled to 18 � C and induced overnight with the addition of 0.35 mM isopropyl �-d-1-thiogalactopyranoside (IPTG).Cells from a 6 l culture were resuspended in phosphate-buffered saline (PBS) supplemented with 400 mM KCl (binding buffer).The resuspended cells were lysed through a combination of lysozyme and high pressure using a French press.The lysate was centrifuged for 1 h at 100 000g at 4 � C and the supernatant was passed through a self-packed column of 5 ml Ni-NTA resin (Qiagen) preequilibrated with binding buffer.The resin was then washed with five column volumes of binding buffer to wash off unbound protein.The column was further washed in two steps with binding buffer supplemented with 10 and 25 mM imidazole.The protein was eluted from the column using elution buffer (1� PBS supplemented with 400 mM KCl and 300 mM imidazole).The eluted protein was dialyzed in two changes of binding buffer at 4 � C to remove excess imidazole.The dialyzed protein was then concentrated to 21 mg ml À 1 and buffer-exchanged into a buffer consisting of 50 mM Tris pH 7.4, 100 mM NaCl (cross-linking buffer).The purity of the enzyme was verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Ubiquitin with a C-terminal glycine-to-cysteine mutation (Ub G76C ) was cloned into a pRSET-A vector and transformed into E. coli strain BL21(DE3).The E. coli BL21(DE3) cells were grown in LB medium at 37 � C to an OD 600 of 0.6, cooled to 18 � C and induced overnight with the addition of 0.35 mM IPTG.Cells from a 3 l culture were resuspended in 50 mM sodium acetate pH 4.5 (buffer A).The resuspended cells were initially lysed with lysozyme and by heating at 80 � C for 30 min.The lysate was centrifuged for 1 h at 100 000g at 4 � C and the supernatant was passed through a self-packed column of SP Sepharose Fast Flow resin (GE Healthcare) that had been pre-equilibrated with five column volumes of buffer A. After collecting the flowthrough, the column was washed with an additional five column volumes of buffer A to remove any unbound protein.To elute the bound protein, buffer A supplemented with increasing amounts of NaCl ranging from 100 mM to 1 M was used as a gradient to separate it from other potential contaminants.The fractions containing pure Ub G76C , confirmed by SDS-PAGE, were pooled, concentrated and buffer-exchanged into 0.1 M sodium phosphate buffer pH 8 (DTNB reaction buffer).
For SdeA DUB (Puvar et al., 2019), UchL1, UchL3, Zup-1, MINDY-1, LotA, LotB, ElaD and ChlaDUB2, E. coli BL21(DE3) cells were grown in LB medium at 37 � C to an OD 600 of 0.6-0.8,cooled to 18 � C and induced overnight with the addition of 0.35 mM IPTG.The cells from a 6 l culture were resuspended in binding buffer.The resuspended cells were then lysed using a combination of lysozyme and and high pressure using a French press.The lysate was centrifuged for 1 h at 70 000g at 4 � C and the supernatant was passed through a self-packed column of 5 ml Glutathione Sepharose resin (GE Healthcare) pre-equilibrated with binding buffer.The resin was then washed with 20 column volumes of binding buffer to wash off unbound protein.The bound fusion proteins were eluted with GST elution buffer (binding buffer with the addition of 10 mM reduced glutathione).The protein was dialyzed against two changes (4 l each) of GST dialysis buffer at 4 � C, with the addition of PreScission protease, to cleave off the GST tag.The dialyzed protein was then passed through the GST column to remove cleaved GST and PreScission protease from the protein solution.The proteolyzed protein was then further purified by size-exclusion chromatography to remove any residual GST.The purified sample was then concentrated, aliquoted and stored at À 80 � C. The purity of the enzymes from every stage of expression and purification were monitored by SDS-PAGE.
For OTULIN, E. coli BL21(DE3) cells were grown in LB medium at 37 � C to an OD 600 of 0.6-0.8,cooled to 16 � C and induced overnight with the addition of 0.35 mM IPTG.Cells from a 6 l culture were resuspended in PBS supplemented with 400 mM KCl (binding buffer).The resuspended cells were lysed using a combination of lysozyme and high pressure using a French press.The lysate was centrifuged for 1 h at 100 000g at 4 � C and the supernatant was passed through a self-packed column of 5 ml Ni-NTA resin (Qiagen) preequilibrated with binding buffer.The resin was then washed with five column volumes of binding buffer to wash off unbound protein.This column was further washed with binding buffer supplemented with 10 and 25 mM imidazole.The protein was eluted from the column using elution buffer (1� PBS supplemented with 400 mM KCl and 300 mM imidazole).The eluted protein with SENP2 protease was dialyzed in two changes of binding buffer at 4 � C to remove excess imidazole.The dialyzed protein was then passed through the Ni-NTA resin to remove the His-SUMO tag.The proteolyzed protein was concentrated and buffer-exchanged into a buffer consisting of 50 mM Tris pH 7.4, 100 mM NaCl (cross-linking buffer).The purity of the enzyme was verified by SDS-PAGE.

Disulfide cross-link screening
Disulfide cross-linking was based on a previously described procedure (Pruneda et al., 2016).A 10 mM stock of 5,5 0 -dithiobis(2-nitrobenzoic acid) (DTNB) was made by dissolving DTNB in DTNB reaction buffer.Ubiquitin G76C was mixed with resuspended DTNB at a final reaction concentration of 250 mM ubiquitin and 2 mM DTNB overnight at 4 � C to form 2-nitro-5-thiobenzoic acid (TNB)-labeled ubiquitin (Ub 76 -TNB).The Ub 76 -TNB was buffer-exchanged into cross-linking buffer to remove excess DTNB and release 2-nitro-5-thiobenzoic acid from the solution.The buffer-exchanged Ub 76 -TNB was mixed with different DUBs at an equal molar ratio in cross-linking buffer and left to react for 1 h at room temperature with shaking end over end.The resulting complex was subsquently buffer-exchanged in cross-linking buffer to remove the released TNB.The formation of cross-linking was monitored via SDS-PAGE.

Crystallization
Purified SdeA DUB was mixed with Ub G76C -TNB adduct in a 1:2 molar ratio and allowed to incubate overnight at 4 � C.After this, the complex was purified by cation-exchange chromatography (Mono Q, GE Healthcare) followed by sizeexclusion chromatography (Superdex 75, GE Healthcare).The purified complex was concentrated to 30 mg ml À 1 and crystallized in hanging drops consisting of 2.8 M sodium acetate-HCl pH 7 after 18 days at room temperature.A complete data set was collected at 2.81 A ˚resolution from a single crystal on the NE-CAT beamline 24-ID-E (� = 0.97918) at the Advanced Photon Source (APS), Argonne National Laboratory.
OtDUB 1-259 disulfide was mixed with Ub G76C -TNB (in a nearly 1:1 molar ratio) and left overnight at 4 � C. The reaction mixture was concentrated to 36 mg ml À 1 and used directly in crystallization without further purification steps.Crystals were grown by the hanging-drop vapour-diffusion method at 20 � C in crystallization buffer consisting of 0.16 M magnesium chloride, 0.08 M Tris pH 8.5, 24% PEG 8000, 20% glycerol after one day at room temperature.To confirm that the crystals obtained were of the disulfide complex, crystals were dissolved in both 5� reducing and 5� nonreducing SDS loading dye and analyzed using SDS-PAGE.A complete data set was collected to 1.85 A ˚resolution from a single crystal on the NE-CAT beamline 24-ID-C (� = 0.97918) at the APS, Argonne National Laboratory.

Structure determination
Diffraction data sets were collected at the APS, Argonne National Laboratory and were processed using HKL-2000 (Otwinowski & Minor, 1997).The structure of SdeA DUB disulfide-linked with Ub G76C was determined by maximumlikelihood molecular replacement using Phaser (McCoy et al., 2007) within the Phenix suite (Liebschner et al., 2019).The structure was then solved by molecular replacement using the SdeA DUB (Sheedlo et al., 2015) structure (PDB entry 5crb) and ubiquitin (Vijay-kumar et al., 1987; PDB entry 1ubq) as models.The asymmetric unit contained two molecules, which were SdeA DUB and Ub G76C .The structure was subjected to multiple steps of model building with Coot (Emsley et al., 2010) and refinement with Phenix, which resulted in a final structure at 2.81 A ˚resolution (Table 1).The final structure was validated using MolProbity (Chen et al., 2010) and deposited in the Protein Data Bank.
The OtDUB-Ub G76C crystal data were indexed, integrated and scaled using X-ray Detector Software (XDS; Kabsch, 2010), AIMLESS (Evans & Murshudov, 2013) and multiple programs from the CCP4 suite (Agirre et al., 2023), all integrated into the RAPD auto-processing program at NE-CAT, in the orthorhombic space group P22 1 2 1 .The structure was solved by molecular replacement using the native OtDUB structure (Berk et al., 2020; PDB entry 6ups) and ubiquitin (Boudreaux et al., 2010; PDB entry 1ubq) as models.The asymmetric unit consisted of one OtDUB molecule and one ubiquitin molecule.Sequential rounds of model building with Coot (Emsley et al., 2010) and refinement with Phenix were used to arrive at the final structure, which was then validated using MolProbity and deposited in the Protein Data Bank.

The reactivity of a panel of DUBs towards Ub G76C shows significant variability
Hydrolytic release of Ub from a target lysine involves nucleophilic attack of the catalytic cysteine of DUB at the isopeptide bond at Gly76 of Ub that links the lysine-bearing leaving group (Boudreaux et al., 2012).Crystal structures of DUBs have shown that the C-terminal Gly75-Gly76 segment of Ub is held in a narrow cleft in the active-site area where the amide carbonyl of Gly76 is positioned within attacking distance of the catalytic cysteine (Boudreaux et al., 2010).This conserved stereochemical feature enables DUBs to recognize and precisely cleave the target isopeptide bond after the diglycine motif.Taking advantage of this unique DUB-Ub recognition feature, we introduced a cysteine residue in place of Gly76 in Ub for engagement with the catalytic cysteine of DUB.However, the placement of this substituent may hinder binding through steric effects.To test the feasibility of cysteine placement at this location for disulfide chemistry, we used a panel of cysteine DUBs from different families, including some recently described prokaryotic examples (Pruneda et al., 2016;Hermanns & Hofmann, 2019), and screened for their ability to produce a disulfide adduct with the Ub cysteine mutant (Fig. 1a).For efficient disulfide formation, Ub G76C was reacted with Ellman's reagent (DTNB) to produce a disulfidelinked 2-nitro-5-thiobenzoic acid (TNB) adduct (Lorenz et al., 2016) and, after washing off the excess reagent, the adduct was treated with each DUB in our list (Fig. 1b).Within an hour at room temperature, some DUBs showed a robust reaction, producing a distinct band on nonreducing SDS-PAGE corresponding to a ubiquitin adduct, while others showed no discernible activity.For example, the Legionella OTU DUB LotB (Ma et al., 2020) showed a robust reaction (showing two closely migrating adduct bands), whereas LotA (Warren et al., 2023), another OTU DUB from the same organism, produced no detectable band.On the other hand, the CE-clan DUB from the same organism, SdeA DUB, reacted readily with the ubiquitin mutant, whereas another CE-clan DUB from Chlamydia trachomatis (CDub2; Hausman et al., 2020) showed no reaction.OTULIN (Keusekotten et al., 2013) and MINDY (Kwasna et al., 2018), two eukaryotic DUBs, produced smearing in the nonreducing gel, preventing a clear analysis of their disulfide product (Fig. 1c).Overall, these results indicate that disulfide formation with Ub G76C can vary widely across different DUBs.

Structure of the SdeA DUB domain in complex with ubiquitin captured by disulfide chemistry
Since SdeA DUB showed robust disulfide formation, we sought to crystallize the disulfide complex to characterize the binding and compare it with previously solved crystal structures of the same DUB with the Ub electrophile inhibitor ubiquitin vinyl methyl ester (Ub-VME; PDB entry 5cra; Sheedlo et al., 2015).The disulfide adduct was purified by ionexchange chromatography (using buffers without a reducing agent) and subjected to crystallization trials (Fig. 2a).Crystals obtained from a condition lacking any reducing agent diffracted to 2.83 A ˚resolution and belonged to space group P3 2 21 (see Section 2; PDB entry 8efw).The structure was solved using molecular replacement (MR) with SdeA DUB and wild-type Ub as search models, assuming that the crystals belonged to the two-protein complex.The MR search yielded 1047 a solution that contained the expected 1:1 complex of the DUB and Ub.A difference electron-density (F o À F c ) map calculated after the MR search showed residual electron density extending from the DUB catalytic cysteine, which we interpreted to correspond to the disulfide bond linking the cysteine residue of Ub G76C .Further refinement after rounds of rebuilding and model adjustment allowed the placement of a disulfide bond into this density (Fig. 2b).
Apart from local distortion of the SdeA DUB near the catalytic cysteine (discussed below), the structure of the complex showed striking similarity to the Ub-VME-bound structure and the Ub product-bound structure (Fig. 2b and Supplementary Fig. S1).The C-terminal tail of Ub G76C is held in the active-site cleft through several backbone hydrogen bonds, most of which remain the same compared with the other two structures (Fig. 3a and Supplementary Fig. S4a).The free carboxylate at Cys76 points towards the catalytic histidine, much like the same group of Ub in the product-bound complex (Supplementary Fig. S2a).The tail, while in a �-sheet arrangement with two strands from either side of the cleft, is embraced from the top by a Glu-Ser hydrogen bond which has previously been noted to behave as a flap that opens and closes during substrate binding and product release (Fig. 3b).This observation indicates that the cysteine mutation at Gly76 does not prevent the entry of the C-terminal Ub tail into the active-site area when the flap opens.Distal from the active site, the interactions with Ub remained largely the same compared with the other two structures.For example, Gln40 of ubiquitin makes a hydrogen-bond network with Tyr33 and Asp61 of SdeA, and Leu8 forms hydrogen bonds to the backbone and side chain of Ser29 (Fig. 3c and Supplementary Fig. S4a).

Disulfide bonding to catalytic cysteine is accompanied by local distortion to accommodate the disulfide bridge
The C � atom of Cys76 of the Ub G76C mutant closely overlaps with the same atom of Gly76 in the Ub product-bound structure, with the free carboxylates pointing down in the same direction towards the catalytic histidine, as alluded to previously (Supplementary Fig. S2a).The thiol group of Ub G76C points up towards the empty space in the active-site cavity, which appears to pull the DUB catalytic cysteine away from its preferred position as observed in the Ub-VME-bound structure (Fig. 2d).The alanine side chain of the SdeA DUB Cys118Ala mutant (the Ub product-bound complex was crystallized with the SdeA DUB catalytic cysteine mutated to alanine) also points in the same direction as the cysteine in the Ub-VME-bound structure.The movement of the catalytic cysteine by �4.6 A ˚(C � -C � distance) to engage with the cysteine of Ub in a disulfide bond induces a local conformational rearrangement, which seems to be necessary, partly due to having a substituent on the C � atom of residue 76 and partly due to the aforementioned relocation of the DUB catalytic cysteine (Fig. 2d and Supplementary Fig. S2b).The resulting disulfide adopts a somewhat standard geometry characterized by an S-S torsion angle of À 70 � .This adjustment to accommodate the disulfide bridge causes the SdeA DUB backbone from residues 114 to 122 to adopt a different position with respect to the other two structures, which overlap nearly perfectly with each other (Supplementary Fig. S2b).The cost of this rearrangement is the unfurling of one hydrogen-bonding interaction in the SdeA DUB active-site area, at the N-terminal end of the helix carrying the catalytic cysteine (Fig. 2d and Supplementary Fig. S2c).Thus, the disulfide complex shows that the interactions of SdeA DUB with Ub largely remain the same, except for a local distortion involving the active-site cysteine.

Crystal structure of OtDUB disulfide-linked to the ubiquitin G76C mutant
To further study the effectiveness of disulfide chemistry to characterize DUB binding interfaces, we decided to use the bacterial effector OtDUB, which has recently been crystallized with Ub in a noncovalent complex (Berk et al., 2020).The Ub G76C -TNB adduct described previously was mixed with OtDUB in near-equimolar proportions and left overnight at room temperature, which yielded approximately 79% conversion, and the mixture was subjected to crystallization without further purification (Supplementary Fig. S3 and Fig. 4b).A sample of the reaction mixture used for crystallization showed a single Ub adduct on nonreducing SDS-PAGE, while a portion of the OtDUB protein sample remained unmodified along with some Ub G76C derivative.The OtDUB construct, unlike SdeA DUB (which only contains the catalytic cysteine), contains two additional cysteines (Cys111 and Cys116) beside the catalytic cysteine (Cys135), all of which are present in the core catalytic domain of the protein.
By adding the Ub reagent in a limited stochiometric amount, we expected to capture a disulfide adduct involving primarily the catalytic cysteine.
Even though the protein sample remained as a mixture of at least three different species (Supplementary Fig. S3), crystallization trials successfully produced crystals that diffracted to 1.85 A ˚resolution.The crystals belonged to the orthorhombic space group P22 1 2 1 (Table 1), with one complex comprising an OtDUB molecule and an Ub molecule in the asymmetric unit.
The structure was solved by molecular replacement using OtDUB and Ub as two separate search models (see Section 2).The MR solution revealed a model of the asymmetric unit that contained a Ub next to an OtDUB, but with its C-terminus pointing away from the catalytic cysteine residue and with no disulfide linkage to any other cysteine in the same OtDUB chain (Fig. 4a; PDB entry 8efx).Instead, the Ub C-terminal tail points towards a symmetry-related OtDUB neighbor, approaching its Cys116 (Fig. 5a).(In OtDUB, this cysteine is nearly 10 A ˚from the catalytic cysteine.)However, the C-terminal tail of Ub G76C was disordered after Arg72, with missing density for the side chains of Leu73 and Arg74, even though the backbone is traceable up to Gly75 (Fig. 5b).While the terminal cysteine residue could not be placed due to missing density, the proximity and orientation of the C-terminus indicated that Ub G76C could be disulfide-linked to Cys116 of the symmetry-related neighbor.Alternatively, despite the substantial population of a disulfide complex in solution, we might have managed to crystallize a noncovalent complex formed from the unreacted OtDUB and Ub G76C species remaining in the reaction mixture.In probing this further, we harvested crystals from the same batch as used for diffraction, carefully washed them and subjected them to SDS-PAGE under either reducing or nonreducing conditions.Under the latter conditions, a single species corresponding to a covalent complex was detected, while the same dissolved crystals produced two distinct bands under reducing conditions (in the presence of DTT) corresponding to separated OtDUB and Ub chains, as expected for a disulfide-linked adduct (Fig. 5c).Within the complex representing the asymmetric unit, OtDUB makes only a handful of contacts with Ub, leaving most of its canonical interaction patches (the widely used Ile44 and Ile36 patches as well as the C-terminus) unsatisfied.The few contacts with Ub in the asymmetric unit complex are provided entirely by four residues in the ubiquitin-binding domain (UBD; see below), but from a face diametrically opposite to a patch of residues known to be responsible for the high-affinity interaction with Ub (Berk et al., 2020;Fig. 4a).The intra-asymmetric unit contacts between the UBD and Ub utilize Lys33 and the backbone atoms of the Gly35-Glu36 segment of Ub and Asn195, Arg196, Lys237 and Glu238 of OtDUB (Fig. 4c).

Crystallographic contacts mediated by high-affinity interaction between
Ub and OtDUB UBD.The likely intersubunit disulfide in the crystals prompted us to further examine the crystallographic contacts (Fig. 6a).In the previously characterized noncovalent complex that was produced under conditions of excess Ub, OtDUB showed three distinct Ub-binding sites: two of them belong to the catalytic domain, with Ub occupying the S1 and S2 sites (Fig. 6b; the S1, S2 notation follows a similar nomenclature as that used to describe protease-substrate interactions, except that the S1, S2 etc. sites refer to the binding pocket for an entire Ub in the context of a polyubiquitin substrate; the first Ub preceding the scissile peptide bond binds at the S1 site, the Ub before that at the S2 site and so on), whereas the third Ubbinding site is contributed solely by a C-terminal extension after the catalytic domain.The extension, consisting of four �-helices, folds into a distinct domain that has been shown to function as an accessory module for recruiting Ub (accordingly, it is referred to as the ubiquitin-binding domain; UBD) through interactions amounting to single-digit nanomolar affinity (Berk et al., 2020).The ubiquitin-binding site on the UBD does not fit the description of a substrate-like arrangement of Ub binding as represented by the Si or Si 0 notation (i = 1, 2, 3 and so on).Moreover, this domain seems to fold independently of the catalytic domain and retains high-affinity Ub binding even as a separate construct outside the context of the OtDUB protein (Berk et al., 2020).
The high-affinity UBD patch, while unsatisfied within the asymmetric unit complex, interacts with Ub from a different symmetry mate using all of the expected Ub-UBD interactions that constitute the high-affinity binding (Fig. 6c).For example, the Ile44 patch on Ub G76C (residues Leu8, Ile44 and Val70) makes close contact with a complementary hydrophobic patch of OtDUB UBD (Val203, Phe207 and Leu221), while electrostatic attraction brings Lys6, Arg42 and His68 of UbG76C into close contact with three aspartates (Asp204, Asp208 and Asp226) of UBD located within the high-affinity patch.The Ile44 interaction with the UBD hydrophobic patch brings interacting hydrophobic residues into close van der Waals contact (Supplementary Fig. S4b).Thus, the Ub-UBD high-affinity interaction provides crystal-packing contacts that seem to be important for crystal growth along one of the axes of the unit cell.

Discussion
Disulfide cross-linking has been used in the past to obtain structures comprising two or more proteins.In the ubiquitin field, this has been used to capture at least six Ub-bound structures of ubiquitin-conjugating enzymes and E3 ligases (Lorenz et al., 2016;Wiener et al., 2012;Maspero et al., 2013;Kamadurai et al., 2013;Ja ¨ckl et al., 2018;Puvar et al., 2020).
In these examples, the disulfide link involves the catalytic cysteine of the ubiquitin-conjugating E2 enzyme or a HECTfamily ubiquitin E3 ligase and the cysteine residue of the G76C Ub mutant.Here, we explore whether a similar strategy can be used to capture Ub bound to cysteine-protease DUBs.Using a panel of eukaryotic and prokaryotic DUBs, we find that the disulfide reactivity with Ub G76C can show significant variation.In some cases, the reaction can proceed to a substantial degree, allowing the isolation of a single-Ub adduct of the disulfide-linked DUB for structural characterization, as exemplified by the CE-clan SdeA DUB from L. pneumophila, which contains a single cysteine as its catalytic residue.LotB, an OTU family DUB from the same organism, reacts readily but produces two distinct apparently single-Ub adducts.These species could result from the reaction of one Ub G76C group with either the catalytic or another reactive cysteine.Previous studies on LotB (also known as Ceg23) have shown that in addition to the catalytic cysteine there is still at least one other reactive cysteine (Ma et al., 2020), which could explain our observation of two distinct single-Ub adducts (Fig. 1c).To the extent that the disulfide reaction is a proximity-driven effect, the observation of multiple disulfide modifications may indicate the presence of additional Ub-binding sites other than the expected S1 site.
While the previous structures of Ub bound to SdeA DUB (Sheedlo et al., 2015(Sheedlo et al., , 2021) ) had many similarities to the disulfide-linked structure obtained in this study, this new structure shows a different arrangement of the catalytic Interactions of UBD and Ub as packing contacts in the crystals.(a) The previously solved OtDUB structure in complex with three Ub molecules shown for comparison (PDB entry 6upu).OtDUB is depicted in ribbon representation and Ub is shown as a surface rendition.(b) The OtDUB-Ub G76C complex (magenta and orange) in the asymmetric unit with two of its symmetry-related OtDUB molecules flanking Ub: OtDUB Sym1 (cyan) and OtDUB Sym2 (lime green).(c) Residues from the UBD domain of OtDUB Sym2 interact with the Ile44 patch of Ub G76C of the asymmetric unit complex.cysteine and some nearby residues in the DUB active site (Fig. 2d and Supplementary Figs.S2b and S2c).Compared with the Ub-bound noncovalent complex (representing binding of the Ub product) and the Ub-VME-bound structure, in which the electrophile warhead mimics the last glycine and the scissile peptide bond in a more or less isosteric arrangement, reaction with Ub G76C results in local distortion at the active-site cysteine, presumably to accommodate the nonisosteric cysteine substitution at Gly76 (Supplementary Fig. S2b).In fact, this substitution may prevent the Ub mutant binding to the DUB in the first place, which could explain the lack of reaction with some of the other DUBs in our panel.It seems likely that SdeA DUB and those that readily react with the Ub mutant may possess an active site that is flexible enough to accommodate the cysteine substitution.The activesite cysteine in the disulfide-linked structure adopts an unproductive orientation which lies beyond an interacting distance from the catalytic histidine (S � -N � distance of 7.7 A ˚). Thus, it appears that disulfide formation occurs when the cysteine is in a more accessible conformation while pointing away from the catalytic histidine, even though it is at the expense of the interaction that is observed in productive cysteine proteases (typically characterized by an S � -N � distance of �4 A ˚).As an implication for structural analysis, the disulfide-linked structure on its own may not yield a productive arrangement of the catalytic residues of the DUB.
In contrast to SdeA DUB, the disulfide reaction of OtDUB with Ub76C resulted in an adduct that involves a different cysteine to the catalytic residue (Fig. 5).It is possible that some amount of catalytic cysteine-linked disulfide adduct could be produced in the reaction but did not yield crystals.Since we went straight from the reaction to crystallization without purification, our results may not entirely reflect the situation in solution.Nevertheless, the crystals were disulfidelinked, based on the behavior of the dissolved crystals under reducing conditions (Fig. 4c), and the disulfide did not involve the catalytic cysteine but instead involved a surface-exposed cysteine.
Under conditions of a limiting concentration of the Ub reagent, it is possible that the nanomolar UBD-binding site becomes saturated first and it is this Ub-bound species that undergoes disulfide chemistry.The C-terminal tail of this Ub connected to the UBD in one complex may favor an 'intermolecular' reaction with a readily accessible cysteine of a different OtDUB-Ub complex in solution (over an 'intramolecular' reaction with the catalytic cysteine, for example, within the same complex), giving rise to the crystals that we have characterized here.In retrospect, it might have been possible to capture the S1 binding site through disulfide linkage only if we had used an excess of the Ub reagent.
The two examples of disulfide strategy described here illustrate the potential limitations of this approach compared with co-crystallization with Ub C-terminal electrophiles.In spite of requiring more steps in their production, in contrast to the simpler cysteine mutagenesis involved in the disulfide strategy, the Ub electrophiles are more likely to produce biologically relevant complexes.

Figure 1
Figure 1 Disulfide-bridge formation as a strategy to capture disulfide-linked DUB-Ub structures.(a) A list of the DUBs tested for disulfide reaction.Eukaryotic and prokaryotic DUBs are grouped according to their families.(b) A schematic of Ub G76C (yellow) disulfide-bridge formation with a target DUB (orange) utilizing DTNB.(c) SDS-PAGE analysis under nonreducing conditions of the reaction mixtures of DUBs with the Ub G76C -TNB adduct.The red boxes indicate examples of DUBs reacting with the Ub reagent.

Figure 2
Figure 2Structure of the disulfide-linked SdeA DUB-Ub G76C complex.(a) SDS-PAGE (nonreducing) analysis of a purified sample of SdeA DUB in a disulfidelinked complex with Ub G76C .(a) Ribbon representation of the crystal structure of the disulfide-linked complex of Ub G76C (slate) and SdeA (raspberry).Right: enlargement of the boxed region shows that there is continuous electron density (2F o À F c at 1�) between the C-terminal cysteine of Ub G76C and the catalytic cysteine of SdeA DUB.(c) Catalytic triad of SdeA DUB compared between the disulfide-linked structure, the Ub-VME-bound structure (lime green) and the apo structure (PDB entry 5crb, C118A mutant; orange).(d) Superposition of the Ub-VME-bound structure (green) and the disulfide-linked SdeA DUB-Ub complex structure (raspberry).The G76C residue is highlighted in stick representation in slate.The movement of the catalytic cysteine and the unfurling of a helical turn (that carries the catalytic cysteine) to accommodate the disulfide bridge between Cys76 of Ub and Cys118 of SdeA is shown by an arrow.The cysteine (green sticks) in the SdeA DUB-Ub-VME complex represents its native-like conformation.

Figure 3
Figure 3 Interactions with Ub are preserved in the disulfide-linked structure.(a) Superposition of the SdeA DUB complex with Ub in the disulfide-linked form (raspberry and slate), the Ub-VME-bound form (green and yellow) and the Ub product-bound form (pink and orange).The C-terminal tail of Ub (from Leu71 to Gly75) is shown as sticks.Active-site SdeA DUB residues that form �-sheet-like interactions with the tail are shown as raspberry sticks.The hydrogen-bond network formed between the C-terminal tail of Ub and SdeA DUB is indicated as dashed lines.The red boxes indicate the regions depicted in (c).The numbering of the boxes corresponds to the numbering in (c).(b) Left: the hydrogen bond (dashed line) between SdeA DUB residues Glu9 and Ser62 is conserved in the VME-bound, product-bound and disulfide-bound structures.Right: in the apo form of SdeA DUB (orange) this interaction is not observed, indicating an open active site.(c) Hydrogen-bonding interactions (dashed lines) in other areas distal to the active site that were observed between Ub and SdeA DUB in the Ub-VME-bound and product-bound structures are maintained in the disulfide-linked structure.

1051 Figure 4
Figure 4 Structure of the disulfide-linked OtDUB-Ub complex.(a) Ribbon representation of the asymmetric unit of the crystal structure of Ub G76C (orange) in complex with OtDUB (magenta) superimposed on the apo OtDUB structure (PDB entry 6upw, olive).The C-terminal tail of Ub is indicated by a red box.The blue box highlights the Ub-binding patch depicted in (c).(b) SDS-PAGE (nonreducing) analysis of the protein solution utilized for crystallization.(c) Residues from the UBD in OtDUB that interact with Ub residues enclosed in the blue box in (a) are shown in stick representation.Hydrogen bonds are indicated as dashed lines.

Figure 5 A
Figure 5 A likely disulfide bond connects OtDUB to ubiquitin from a symmetry-related counterpart.(a) A symmetry-related molecule of OtDUB (OtDUB Sym1 , cyan) located proximal to the C-terminal tail of Ub from the asymmetric unit.Cys116 is shown in stick representation (orange).(b) An enlarged view of the boxed region showing the residues and the corresponding electron density (2F o À F c at 1�) of the C-terminal end of Ub G76C and a polypeptide segment around Cys116 of OtDUB.(c) Samples of the crystals were run on an SDS-PAGE gel after dissolving them in both nonreducing (NR) and reducing (R) SDS loading buffers.

Table 1
Data-collection and processing statistics.Values in parentheses are for the outer shell.